Join us for a TIDES USA Investor Recap Call – May 22, 2025, at 8 am ET
Step into the future of RNAi
Step into the future at TIDES USA. We're excited to see you there! Whether you're stopping by booth 619, attending our TIDES talk, or joining us for our main conference session, you'll hear from our experts about how we're pioneering scalable and sustainable RNA manufacturing with enzymatic synthesis.
Spotlight Presentation
Title: Oligonucleotide CMC for Sustainable and Scalable Enzymatic siRNA manufacturing
Reason to attend: This presentation will showcase the impurity profile, product yield, and output reproducibility of different enzymatic processes and compare these approaches to phosphoramidite chemistry-based oligo synthesis.
When: Wednesday, May 21, 9:30-10:00am
Where: Main stage for CMC track (room TBD)
About the Speaker
Alison Moore, PhD, Chief Technical Officer
Learn more about the speaker and abstract below.
TIDES Talk
Title: Comparative Analysis of Diastereomeric Distribution in siRNA Synthesis: PAC vs Enzymatic Synthesis
Reason to attend: This study compares the diastereomeric profiles of siRNA oligonucleotides synthesized via phosphoramidite chemistry versus the Enzyme Catalyzed Oligo ECO Synthesis™️ manufacturing platform utilizing 3′-phosphate blocked ribonucleotides (NQPs). We explore the impact of PS modifications and their position within the oligonucleotide sequence on the resulting diastereomeric composition of siRNA synthesized utilizing both methods.
When: Wednesday, May 21, 10:10-10:20am
Where: Exhibit Hall
About the speaker
Stephanie Forget, Senior Scientist, Protein Engineering
Learn more about the talk and speaker below.
Poster
Title: Machine Learning-Guided Ligation Fragment Design for Efficient siRNA Synthesis
Reason to attend: The growing demand for siRNA therapeutics calls for scalable, efficient manufacturing methods. Engineered dsRNA ligases enable high-yield assembly of siRNA from short RNA fragments, with performance dependent on both ligase selection and fragment design. We present an AI/ML-guided approach to optimize fragment design, improving ligation efficiency and enabling one-pot assembly with >90% success. This method reduces the need for broad ligase screening, accelerating the path to scalable siRNA production.
When: TBD
Where: TBD
About the Speaker
Mathew Miller, Director, Life Sciences and RNA Technologies
Spotlight Presentation with Bachem
Title: Efficient Large-scale siRNA Manufacturing: Enzymatic Ligation of Short RNA Fragments
Reason to attend: Linear synthesis by continuous-flow SPOS is the current state-of-the-art technology to manufacture oligonucleotide APIs. A very promising alternative, especially to manufacture large quantities, is the enzymatic ligation of short oligonucleotide fragments. This approach offers several advantages compared to the conventional strategy like higher yields and reduced purification burdens. Bachem and Codexis will present a case study focusing on a therapeutically relevant siRNA sequence and benchmark different technologies used to manufacture siRNA fragments. We will discuss a wholistic view of the manufacturing process and review control strategies for residual enzyme.
When: Tuesday, May 20, 12:05-12:35pm
Where: TBD
About the Speaker
Arne Berthelmann, Senior Director Oligonucleotide R & D
Learn more about the talk and speaker below.
Visit booth 619
Why stop by booth 619? Aside from speaking with our team of RNA manufacturing experts and commercial team members grab a fun giveaway while supplies last.
New to Codexis? Learn more below.
About Us
Enabling Manufacturing of Therapeutic siRNA
When manufacturing siRNA for therapeutic applications, Codexis delivers high yields and minimizes unwanted byproducts, while being economically sound. We have a suite of enzymatic pathways to synthesize siRNA, so that you can choose an option that best meets your needs.
Why Codexis?
- dsRNA Ligase Screening and Optimization Services to Complement Solid State Synthesis
- In-house Ligation Capabilities for GLP-grade siRNA
- COMING SOON: ECO Synthesis Innovation Lab for Custom Project Development